CRISPR-Cas13a-Based Detection for Bovine Viral Diarrhea Virus
نویسندگان
چکیده
منابع مشابه
Detection of Bovine Viral Diarrhea Virus in Bovine Semen Using Nested-PCR
A rapid and sensitive reverse transcription polymerase chain reaction (RT-PCR) and nested-PCR were used to detect bovine viral diarrhea virus 1 (BVDV-1) in bull semen. Selected primers could amplify a part of the 5´UTR of the BVDV genome. A 294 bp DNA fragment was amplified and specificity of the results was confirmed by direct sequencing of the PCR product. Prior to RNA extraction, the seminal...
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Bovine viral diarrhea virus (BVDV) is a significant pathogen associated with gastrointestinal, respiratory, and reproductive diseases of cattle worldwide. It causes continuous economic losses to the cattle industry primarily due to decreased reproductive performance. The ability of virus to cross the placenta during early pregnancy can result in the birth of persistently infected (PI) calves. P...
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Rapid, inexpensive, and sensitive nucleic acid detection may aid point-of-care pathogen detection, genotyping, and disease monitoring. The RNA-guided, RNA-targeting clustered regularly interspaced short palindromic repeats (CRISPR) effector Cas13a (previously known as C2c2) exhibits a "collateral effect" of promiscuous ribonuclease activity upon target recognition. We combine the collateral eff...
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Bovine viral diarrhea virus (BVDV) is an economically important pathogen of the livestock industry worldwide. BVDV is classified into cytopathic (cp) and noncytopathic (ncp), depending on its effects on cultured cells. BVDV is known to alter the host’s immune response. Of this, major histocompatibility complex (MHC) class II molecules play a central role in the development and function of the i...
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BVDV is estimated to cost an average dairy between $20 and $160 per adult cow per year. These losses include medication and labor to treat acute infections, milk production losses in lactating cows, infertility due to decreased conception and early embryonic death, abortions, loss of weak, abnormal, or persistently infected calves. Large scale herd outbreaks such as occurred in the early 1990s ...
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ژورنال
عنوان ژورنال: Frontiers in Veterinary Science
سال: 2021
ISSN: 2297-1769
DOI: 10.3389/fvets.2021.603919